The Agilent G2258-60007 is a specialized 5ml analytical head assembly designed for use in Agilent high-performance liquid chromatography (HPLC) and automated liquid handling systems, specifically within the G2258A Dual-Loop Autosampler modules.
Product Parameters (Datasheet)
- Model Number: G2258-60007
- Part Name: 5ml Head Ay Tag (Analytical Head Assembly)
- Volume Capacity: 5.0 mL
- Compatibility: Agilent G2258A Dual-Loop Autosampler / Preparative Scale Systems
- Material: High-purity Stainless Steel and Inert Fluoropolymer Seals
- Pressure Rating: Optimized for preparative and semi-preparative pressure ranges
- Connection Type: Standard 10-32 threaded fittings for capillary integration
- Feature: Integrated Electronic ID Tag (Smart Tag) for automated system recognition and stroke counting
Country of Origin
This precision analytical component is manufactured by Agilent Technologies in Germany (formerly part of the Hewlett-Packard analytical division), ensuring world-class metrology and chemical resistance standards.
Application Fields
The G2258-60007 is a critical component for large-volume injection and sample processing in:
- Pharmaceutical Purification: Large-scale isolation of active pharmaceutical ingredients (APIs).
- Proteomics & Metabolomics: Handling bulk biological samples for fraction collection.
- Environmental Testing: Large-volume water analysis where high sensitivity requires larger sample loads.
- Chemical Research: Semi-preparative chromatography for synthetic compound verification.
Product Usage Instructions
- Installation: Power down the autosampler module. Align the analytical head with the drive motor coupling and secure the mounting screws.
- Fluidic Connections: Connect the inlet and outlet capillaries using gold-plated or stainless steel fittings. Ensure no air bubbles are trapped in the 5ml chamber.
- Priming: Perform a “Flush” or “Prime” cycle through the Agilent ChemStation or OpenLab software to remove storage solvents.
- Tag Recognition: Verify that the “Smart Tag” is recognized by the system; this ensures the software applies the correct 5ml volume calibration.
- Maintenance: Periodically inspect the plunger seal for leaks. Replace the seal using the Agilent seal-wash tool if ghost peaks or pressure fluctuations appear.
Product Technical Description
Technically, the G2258-60007 acts as the “syringe” or displacement heart of the autosampler. It utilizes a precision-ground plunger to draw and dispense exactly 5ml of fluid. The “Tag” feature is an embedded RFID or electronic chip that communicates the assembly’s specifications and usage history to the HPLC controller. This prevents user error (such as using 5ml settings on a 100µl head) and tracks the wear of the internal seals based on total stroke cycles, ensuring GLP (Good Laboratory Practice) compliance.
Q&A (Common Questions)
Q: Can I use this head for standard analytical 10µl injections? A: While it can technically perform smaller injections, a 5ml head is optimized for large-volume preparative work. Precision at the micro-liter level is significantly better with a smaller 100µl analytical head.
Q: What should I do if the system doesn’t recognize the “Tag”? A: Clean the electronic contact points on the back of the head assembly with isopropyl alcohol. If recognition still fails, the electronic ID tag may be defective.
Q: Is this assembly compatible with organic solvents like Acetonitrile? A: Yes, the materials (Stainless Steel and specialized seals) are designed to be compatible with most common HPLC mobile phases.
Q: How often should the internal plunger be replaced? A: This depends on the buffer concentration and cleanliness of your samples. Typically, seals should be inspected every 6 to 12 months in high-use environments.
Similar Model Recommendations
- Agilent G2258-60008 (Alternative volume variant)
- Agilent G1313-60010 (Standard 100µl Analytical Head)
- Agilent G1329-60007 (Extended volume head for standard autosamplers)
- Agilent 5063-6503 (Replacement Plunger Seal for 5ml heads)
- Agilent G4220-60007 (High-pressure binary pump head assembly)
For procurement and technical support regarding the Agilent G2258-60007 and other Agilent chromatography parts, please contact our specialized lab equipment team.
